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Hif1atm1Pec
Targeted Allele Detail
Nomenclature
Symbol: Hif1atm1Pec
Name: hypoxia inducible factor 1, alpha subunit; targeted mutation 1, Peter Carmeliet
MGI ID: MGI:2179429
Synonyms: Hif1alpha-, HIF-1alpha-
Gene: Hif1a  Location: Chr12:73901375-73947530 bp, + strand  Genetic Position: Chr12, 31.99 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:76427
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsExon 2 of this gene was replaced with a neomycin cassette via homologous recombination. Northern and Western blot analysis confirmed lack of gene expression in homozygous mutant embryonic stem (ES) cells. (J:76427)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 7 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hif1a Mutation:  11 strains or lines available
Notes
Injection of homozygous ES cells into nude mice results in tumors that have reduced formation of large vessels and that have hypoxic microenvironments, however growth of tumors is not retarded but accelerated due to decreased hypoxia-induced apoptosis and increased stress-induced proliferation. Additionally, hypoxic and hypoglycemic conditions do not reduce proliferation and increase apoptosis in homozygous ES cells as in wild-type ES cells (J:76427).
References
Original:  J:76427 Carmeliet P, et al., Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis. Nature. 1998 Jul 30;394(6692):485-90
All:  14 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/23/2020
MGI 6.15
The Jackson Laboratory