Olig1^tm1(cre)Rth
Targeted Allele Detail

Summary

• Symbol: Olig1^tm1(cre)Rth
• Name: oligodendrocyte transcription factor 1; targeted mutation 1, David H Rowitch
• MGI ID: MGI:2179311
• Synonyms: Cre^Olig1 Tg, Olig1-, Olig1-cre, OLIG1^cre, Olig1-null;PGKneo, Olig1^tm1Rth
• Gene: Olig1 Location: Chr16:91066660-91068821 bp, + strand Genetic Position: Chr16, 52.67 cM
• Alliance: Olig1^tm1(cre)Rth page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75868
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Mutations: Insertion, Intragenic deletion
• Olig1^tm1(cre)Rth expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Olig1 (MGI:1355334)

• Mutation details: The majority of the coding region for this gene was replaced with a cre-neo cassette via homologous recombination so that the cre coding sequence is joined in-frame to the endogenous gene's coding region immediately following the translation initiation site. In situ hybridization of spinal cord from homozygous mutant embryos did not detect transcripts from the endogenous gene. In spinal cord from E10.5-E12.5 embryos heterozygous for this allele and for the conditional (Cre recombinase activated) lacZ reporter allele Gt(ROSA)26Sor^tm1Sor, Xgal staining co-localized with in situ hybridizing mRNAs for motor neuron and oligodendrocyte markers; antibodies to beta-galactosidase co-localized with those to an oligodendrocyte marker, but not to an astrocyte marker. (J:75868)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Olig1 (mouse) Summary of all recombinase alleles driven by Olig1. Comparative matrix view of recombinase activities Alliance mouse Olig1 gene page

Disease models

Expression

In Mice Carrying this Mutation:	104078 assay results 9 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Olig1 Mutation: 13 strains or lines available

References

• Original: J:75868 Lu QR, et al., Common developmental requirement for olig function indicates a motor neuron/oligodendrocyte connection. Cell. 2002 Apr 5;109(1):75-86
• All: 82 reference(s)