Rr158^tm1Cgn
Targeted Allele Detail

Summary

• Symbol: Rr158^tm1Cgn
• Name: regulatory region 158; targeted mutation 1, University of Cologne
• MGI ID: MGI:2179291
• Synonyms: iEkappaT, Igk-Eik^tm1Cgn
• Gene: Rr158 Location: unknown Genetic Position: Chr6, Syntenic
• Alliance: Rr158^tm1Cgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:12574
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance gene cassette controlled by the rabbit beta-globin promoter plus an enhancer element containing four tandem repeats of the GTIIC+GTI motif, which is active only in ES cells, was used in this allele. This cassette replaced the immunoglobulin kappa chain intron enhancer (Ekappa) sequence, located between Igkj and Igkc gene segment clusters, containing the octamer site, the NF-kappaB binding site, kappa E1-E3, and the kappa silencer. (J:12574)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr158 Mutation: 0 strains or lines available

Notes

ES cell line = D3 (129S2/SvPas) or E14.1 (129P2/OlaHsd).

References

• Original: J:12574 Takeda S, et al., Deletion of the immunoglobulin kappa chain intron enhancer abolishes kappa chain gene rearrangement in cis but not lambda chain gene rearrangement in trans. EMBO J. 1993 Jun;12(6):2329-36
• All: 9 reference(s)