Msh4^tm1Wed
Targeted Allele Detail

Summary

• Symbol: Msh4^tm1Wed
• Name: mutS homolog 4; targeted mutation 1, Winfried Edelmann
• MGI ID: MGI:2179034
• Synonyms: Msh4-
• Gene: Msh4 Location: Chr3:153562783-153611495 bp, - strand Genetic Position: Chr3, 78.7 cM
• Alliance: Msh4^tm1Wed page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62204
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 4 of this gene was replaced with a PGK-hygromycin cassette resulting in the introduction of multiple stop codons in the reading frame and loss of the nucleotide binding domain and helix-loop-helix domain. Gene transcripts were not detected in testes of homozygous mutant animals as assayed by Northern blot. Immunolocalization experiments failed to detect protein on or around spermatocyte meiotic chromosomes of homozygous mutant animals. (J:62204)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Msh4 Mutation: 39 strains or lines available

References

• Original: J:62204 Kneitz B, et al., MutS homolog 4 localization to meiotic chromosomes is required for chromosome pairing during meiosis in male and female mice. Genes Dev. 2000 May 1;14(9):1085-97
• All: 6 reference(s)