Gria2^tm1Rsp
Targeted Allele Detail

Summary

• Symbol: Gria2^tm1Rsp
• Name: glutamate receptor, ionotropic, AMPA2 (alpha 2); targeted mutation 1, Rolf Sprengel
• MGI ID: MGI:2178120
• Synonyms: GluR-B^deltaECS
• Gene: Gria2 Location: Chr3:80588757-80710142 bp, - strand Genetic Position: Chr3, 35.5 cM
• Alliance: Gria2^tm1Rsp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:30145
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The editing site complementary sequence (ECS) in intron 11 was replaced with a loxP-flanked neomycin resistance cassette. The neomycin cassette was removed by transient Cre expression in ES cells prior to the production of chimeric mice, leaving a single loxP site in place of the ECS. RT-PCR analysis on RNA derived from brains of homozygous mice demonstrated that unedited transcripts were produced from this allele. (J:30145)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gria2 Mutation: 75 strains or lines available

References

• Original: J:30145 Brusa R, et al., Early-onset epilepsy and postnatal lethality associated with an editing-deficient GluR-B allele in mice. Science. 1995 Dec 8;270(5242):1677-80
• All: 2 reference(s)