Srebf1^tm1Mbr
Targeted Allele Detail

Summary

• Symbol: Srebf1^tm1Mbr
• Name: sterol regulatory element binding transcription factor 1; targeted mutation 1, Michael S Brown
• MGI ID: MGI:2177959
• Synonyms: SREBP-1c -, SREBP-1c null
• Gene: Srebf1 Location: Chr11:60089915-60113407 bp, - strand Genetic Position: Chr11, 37.81 cM
• Alliance: Srebf1^tm1Mbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75320
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An in-frame lacZ gene with a nuclear localization signal was inserted into exon 1c of the gene. A loxP flanked neomycin resistance and Angiotensin-cre cassette (ACN) was inserted following the lacZ gene. The neomycin/cre cassette was autocatalytically removed in the male germline by activation of the Ace promoter in testis. RNase protection assays on liver RNA from homozygous mice demonstrated that the 1c isoform transcript was not detectable, while expression of the 1a isoform was unaffected. Western blot analysis confirmed that the expression of the encoded protein was greatly reduced from this allele; in addition, beta-galactosidase expression was detected from this allele in frozen liver sections. (J:75320)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Srebf1 Mutation: 55 strains or lines available

References

• Original: J:75320 Liang G, et al., Diminished Hepatic Response to Fasting/Refeeding and Liver X Receptor Agonists in Mice with Selective Deficiency of Sterol Regulatory Element-binding Protein-1c. J Biol Chem. 2002 Mar 15;277(11):9520-8
• All: 23 reference(s)