About   Help   FAQ
E2f3tm1Lees
Targeted Allele Detail
Summary
Symbol: E2f3tm1Lees
Name: E2F transcription factor 3; targeted mutation 1, Jacqueline A Lees
MGI ID: MGI:2177435
Synonyms: E2F3-
Gene: E2f3  Location: Chr13:30090558-30170046 bp, - strand  Genetic Position: Chr13, 13.09 cM, cytoband A3.3
Alliance: E2f3tm1Lees page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:65128
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA PGK-neomycin resistance cassette replaced sequences encoding amino acids 134-294, which comprise the nuclear localization signal, cyclin A binding, DNA binding, and the leucine zipper domains, and introduced an in-frame termination codon just upstream of the cassette. Western blot analysis did not detect protein in MEF cells derived from homozygous mutant embryos. (J:65128)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 3 anatomical structure(s)
Tumor Data
List all tumor models in MMHCdb carrying E2f3tm1Lees
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any E2f3 Mutation:  32 strains or lines available
References
Original:  J:65128 Humbert PO, et al., E2f3 is critical for normal cellular proliferation. Genes Dev. 2000 Mar 15;14(6):690-703
All:  15 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/12/2024
MGI 6.23
The Jackson Laboratory