Plin1^tm1Chan
Targeted Allele Detail

Summary

• Symbol: Plin1^tm1Chan
• Name: perilipin 1; targeted mutation 1, Lawrence Chan
• MGI ID: MGI:2176248
• Synonyms: Plin-
• Gene: Plin1 Location: Chr7:79370912-79382652 bp, - strand Genetic Position: Chr7, 45.11 cM
• Alliance: Plin1^tm1Chan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66267
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRES-lacZ gene followed by a neomycin resistance cassette replaced a genomic fragment containing part of exon 2 and intron 2. Northern blot analysis demonstrated that the encoded transcript was not detectable in homozygous mice. Western blot anlaysis on extracts derived from adipocytes and testis of homozygous mice confirmed that no detectable protein is expressed from this allele. (J:66267)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Plin1 Mutation: 30 strains or lines available

References

• Original: J:66267 Martinez-Botas J, et al., Absence of perilipin results in leanness and reverses obesity in Leprdb/db mice. Nat Genet. 2000 Dec;26(4):474-9
• All: 4 reference(s)