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Targeted Allele Detail
Symbol: Akt2tm1.1Mbb
Name: thymoma viral proto-oncogene 2; targeted mutation 1.1, Morris J Birnbaum
MGI ID: MGI:2158456
Synonyms: Akt2-, Akt2 KO, PKBbeta-
Gene: Akt2  Location: Chr7:27591552-27640826 bp, + strand  Genetic Position: Chr7, 15.94 cM, cytoband B1
Germline Transmission:  Earliest citation of germline transmission: J:71491
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsThis allele is a derivative of Akt2tm1Mbb. Cre-mediated recombination in vivo under the control of a 6 kb 5'-flanking sequence from the Pou3f4 gene excised the floxed exons 4 and 5 in the germline. The excision of exons 4 and 5 results in a frameshift mutation that would lead to a premature termination even if the remaining exon 3 were to splice to exon 6. Exon 5 encodes the lysine residue necessary for catalytic activity. Western blot analyses using a polyclonal antibody did not detect protein in liver, muscle, and isolated adipocytes from homozygous mice. (J:71491)
View phenotypes for all genotypes (concatenated display).
Disease models
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Akt2 Mutation:  30 strains or lines available
Original:  J:71491 Cho H, et al., Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta). Science. 2001 Jun 1;292(5522):1728-31
All:  61 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.0
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