Prf1^tm1Clrk
Targeted Allele Detail

Summary

• Symbol: Prf1^tm1Clrk
• Name: perforin 1 (pore forming protein); targeted mutation 1, William R Clark
• MGI ID: MGI:2158403
• Synonyms: perforin-less (-), pfp^-, PKO
• Gene: Prf1 Location: Chr10:61133612-61140459 bp, + strand Genetic Position: Chr10, 32.18 cM
• Alliance: Prf1^tm1Clrk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:21531
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance gene was inserted into exon 2. Western blot analysis on splenocytes derived from homozygous mice demonstrated that no detectable protein is produced from this allele. (J:21531)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Prf1 Mutation: 48 strains or lines available

References

• Original: J:21531 Walsh CM, et al., Immune function in mice lacking the perforin gene. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10854-8
• All: 66 reference(s)