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Il1btm1Dch
Targeted Allele Detail
Nomenclature
Symbol: Il1btm1Dch
Name: interleukin 1 beta; targeted mutation 1, David D Chaplin
MGI ID: MGI:2157804
Synonyms: IL-1beta-, IL-1BtmChaplin
Gene: Il1b  Location: Chr2:129364570-129371139 bp, - strand  Genetic Position: Chr2, 62.97 cM, cytoband F
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:33465
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsA PGK-neomycin resistance cassette was inserted into exon 4 that resulted in an in-frame termination codon upstream of the ICE cleavage site. The authors predict that the receptor-binding domain of the protein would not be translated. Northern blot analysis detected no transcripts in LPS-stimulated peritoneal exudate cells from homozygous mice. Pulse-chase labeling and immunoprecipitation studies of thioglycollate-elicited PEC demonstrated that no detectable protein was present in homozygous mice. ELIZA analysis of ATP-treated PEC also did not detect protein produced by this allele. (J:33465)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Il1b Mutation:  2 strains or lines available
References
Original:  J:33465 Shornick LP, et al., Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone. J Exp Med. 1996 Apr 1;183(4):1427-36
All:  19 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
06/23/2015
MGI 5.22
The Jackson Laboratory