Atp1b2^{tm1(Atp1b1)Mtg}
Targeted Allele Detail

Summary

• Symbol: Atp1b2^{tm1(Atp1b1)Mtg}
• Name: ATPase, Na+/K+ transporting, beta 2 polypeptide; targeted mutation 1, Dirk Montag
• MGI ID: MGI:2157777
• Synonyms: beta2/beta1^ki
• Gene: Atp1b2 Location: Chr11:69490562-69496768 bp, - strand Genetic Position: Chr11, 42.86 cM
• Alliance: Atp1b2^{tm1(Atp1b1)Mtg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50902
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Atp1b2^{tm1(Atp1b1)Mtg} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Atp1b1 (MGI:88108)

• Mutation details: A mouse cDNA containing the sequences encoding amino acids 14 to 304 of the Atp1b1 protein was inserted in frame into exon 1, at codon 18 of the Atp1b2 gene. A loxP flanked neomycin cassette was inserted 3' to the cDNA sequence. Northern blot analysis indicated that a mutant fusion transcript is produced from this allele, and western blot analysis on brain membrane fractions indicated that increased levels of Atp1b1 protein were detected, and expression of Atp1b2 was eliminated in homozygous mice. (J:50902)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atp1b2 Mutation: 23 strains or lines available

References

• Original: J:50902 Weber P, et al., Na,K-ATPase subunit beta1 knock-in prevents lethality of beta2 deficiency in mice. J Neurosci. 1998 Nov 15;18(22):9192-203
• All: 3 reference(s)