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Acetm2Keb
Targeted Allele Detail
Nomenclature
Symbol: Acetm2Keb
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; targeted mutation 2, Kenneth E Bernstein
MGI ID: MGI:2155953
Synonyms: ACE.2 KO
Gene: Ace  Location: Chr11:105967945-105989964 bp, + strand  Genetic Position: Chr11, 68.84 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:40475
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsReplacement of 91bp upstream of the testis specific transcription start site and the first 17bp of the testis specific first exon with a neomycin cassette. All if the somatic coding regions downstream of the neomycin cassette were inserted into intron 12. However, somatic isoforms expressed from this allele do not contain the C-terminal hydrophobic tail. Northern blot and RT-PCR analysis revealed that a cryptic splice site caused a 40bp insertion into the transcript and introduced an in frame stop codon. Western blot and activity assays demonstrated an absence of protein in tissues of homozygous mice. (J:40475)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ace Mutation:  15 strains or lines available
References
Original:  J:40475 Esther CR, et al., The critical role of tissue angiotensin-converting enzyme as revealed by gene targeting in mice. J Clin Invest. 1997 May 15;99(10):2375-85
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/21/2017
MGI 6.08
The Jackson Laboratory