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Targeted Allele Detail
Symbol: Chrm2tm1Jwe
Name: cholinergic receptor, muscarinic 2, cardiac; targeted mutation 1, Jurgen Wess
MGI ID: MGI:2155543
Synonyms: M2-, M2 KO, M2R-, mAChR2-
Gene: Chrm2  Location: Chr6:36388084-36528414 bp, + strand  Genetic Position: Chr6, 15.48 cM
Germline Transmission:  Earliest citation of germline transmission: J:53061
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA genomic fragment corresponding to sequences encoding the N terminus of the third transmemebrane region to the C terminus of the third intracellular loop was replaced with a neomycin resistance cassette. In situ hybridization experiments demonstrated that no transcript was detectable in brain tissue of homozygous mice. In addition, mutant hearts were unable to bind significant amounts of the muscarinic radioligand [3H]QNB, and muscarinic agonist-induced bradycardia was no longer observed. (J:53061)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 4 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Chrm2 Mutation:  20 strains or lines available
Original:  J:53061 Gomeza J, et al., Pronounced pharmacologic deficits in M2 muscarinic acetylcholine receptor knockout mice. Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1692-7
All:  53 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory