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Smad1tm2.1Rob
Targeted Allele Detail
Nomenclature
Symbol: Smad1tm2.1Rob
Name: SMAD family member 1; targeted mutation 2.1, Elizabeth J Robertson
MGI ID: MGI:2155485
Synonyms: Smad1Robcn
Gene: Smad1  Location: Chr8:79338395-79399518 bp, - strand  Genetic Position: Chr8, 37.56 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:71998
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsThis allele was generated by crossing mice carrying the Smad1tm2Rob allele to mice carrying a Cre transgene controlled by a mouse protamine 1 promoter that directs Cre-recombinase expression specifically to the male germline. An in vivo Cre-mediated excision removed all of the first coding exon and approximately 2kb of 5' and 3' flanking genomic sequences, and left a single loxP and EcoRI site in the locus. Northern analysis of 9.5dpc homozygous embryos using a probe for the 3' UTR detected a shorter transcript, but not the wild-type transcript. Western blot analysis using an antibody that recognizes the MH2 domain did not detect intact or truncated protein in 9.5dpc homozygous embryos. (J:71998)
Inheritance:    Recessive
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Expression
In Mice Carrying this Mutation: 35 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Smad1 Mutation:  12 strains or lines available
References
Original:  J:71998 Tremblay KD, et al., Mouse embryos lacking Smad1 signals display defects in extra-embryonic tissues and germ cell formation. Development. 2001 Sep;128(18):3609-21
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/28/2015
MGI 5.22
The Jackson Laboratory