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Apptm1Cwe
Targeted Allele Detail
Nomenclature
Symbol: Apptm1Cwe
Name: amyloid beta (A4) precursor protein; targeted mutation 1, Charles Weissmann
MGI ID: MGI:2154522
Synonyms: beta APP delta
Gene: App  Location: Chr16:84954440-85173707 bp, - strand  Genetic Position: Chr16, 46.92 cM, cytoband C3-qter
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:21937
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsA neomycin resistance cassette was inserted into exon 2 of the gene. Northern blots of brain, kidney, and lung tissues from homozygous mutant mice showed no detectable full-length wild-type mRNA. Two smaller bands were detectable by Northern blot. The authors attributed these bands to transcription through the inserted neomycin cassette and beyond. Further analysis by Western blot of brain extracts from homozygous mutant mice showed no full-length wild-type protein. Again, two bands, which the authors attributed to carboxy-terminal breakdown products of the shortened protein, were seen. The resulting proteins were determined to lack amino acids 20 - 75. (J:21937)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any App Mutation:  10 strains or lines available
References
Original:  J:21937 Muller U, et al., Behavioral and anatomical deficits in mice homozygous for a modified beta-amyloid precursor protein gene. Cell. 1994 Dec 2;79(5):755-65
All:  10 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
06/23/2015
MGI 5.22
The Jackson Laboratory