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Targeted Allele Detail
Symbol: Apptm1Cwe
Name: amyloid beta (A4) precursor protein; targeted mutation 1, Charles Weissmann
MGI ID: MGI:2154522
Synonyms: beta APP delta
Gene: App  Location: Chr16:84952666-85175255 bp, - strand  Genetic Position: Chr16, 46.92 cM, cytoband C3-qter
Germline Transmission:  Earliest citation of germline transmission: J:21937
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
Mutation detailsA neomycin resistance cassette was inserted into exon 2 of the gene. Northern blots of brain, kidney, and lung tissues from homozygous mutant mice showed no detectable full-length wild-type mRNA. Two smaller bands were detectable by Northern blot. The authors attributed these bands to transcription through the inserted neomycin cassette and beyond. Further analysis by Western blot of brain extracts from homozygous mutant mice showed no full-length wild-type protein. Again, two bands, which the authors attributed to carboxy-terminal breakdown products of the shortened protein, were seen. The resulting proteins were determined to lack amino acids 20 - 75. (J:21937)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 6 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any App Mutation:  20 strains or lines available
Original:  J:21937 Muller U, et al., Behavioral and anatomical deficits in mice homozygous for a modified beta-amyloid precursor protein gene. Cell. 1994 Dec 2;79(5):755-65
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory