Emo3^DBA/2J
QTL Variant Detail

Summary

• QTL variant: Emo3^DBA/2J
• Name: emotionality 3; DBA/2J
• MGI ID: MGI:2154418
• QTL: Emo3 Location: Chr1:146130858-146130993 bp Genetic Position: Chr1, Syntenic

Variant origin

• Strain of Specimen: DBA/2J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers decreased emotionality compared to A/J, C3H/HeJ, and I/LnJ. (J:53482)
• Inheritance: Not Specified

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:53482

A behavioral quantitative trait (QTL) identified in mice as Emo for emotionality was measured by the negative correlation of open-field ambulation (OFA) and open-field defaction (OFD). The Emo phenotype was assesed in 751 mice from heterogeneous stock derived from an eight way cross including the following inbred mouse strains: A/J, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6J, DBA/2J, I/LnJ and RIIIS/J and found to be significantly correlated with Chromosome 1 marker D1Mit264 (ANOVA p value 3.1 x 10^-9). To further refine the area containing the QTL the authors used the EUCIB mapping panel, YACs and RI strains. The following markers showed a significant association with the trait: D1Mit394, D1Mit100, D1Mit423, D1Mit198, D1Mit194 and D1Mit102. This allowed the authors to refine the trait to an approximate 0.2 cM region of mouse Chromosome 1, with an estimated cM position of 64.

J:109577

Linkage analysis was performed to identify anxiety and neuroticsm loci in 751 animals from a heterogeneous stock derived from 8 different inbred strains (A/J, AKR, BALB/c, C3H, C57BL/6, DBA, I, and RIII. A QTL interval on mouse Chromosome 1 mapped to 64.84 cM near D1Mit394. This locus is near the previously identified emotionality QTL Emo3 at 73 cM, and the region is syntentic to human Chromosome 1 at 201 cM - 203 cM. Human neuroticism maps to Chromosome 1 at 113 cM - 144 cM.

The QTL peak spans a 0.8 cM(4.78 Mb) interval containing 17 pseudogenes and 9 genes. Sequence analysis revealed conservative coding variants in Glrx2 (77.8 cM), Trove2 (formerly Ssa2; 78.7 cM), and Rgs1 (78 cM). Two other potential candidate genes located in the QTL interval are Rgs2 (78 cM), and Rgs13 (78 cM). Complementation test was performed by crossing Rgs2 knockout animals with high-anxiety or low-anxiety inbred strains. Interaction between Rgs2 strain variant alleles and the knockout allele was observed.

References

• Original: J:53482 Talbot CJ, et al., High-resolution mapping of quantitative trait loci in outbred mice. Nat Genet. 1999 Mar;21(3):305-8
• All: 1 reference(s)