Col2a1^tm1Prc
Targeted Allele Detail

Summary

• Symbol: Col2a1^tm1Prc
• Name: collagen, type II, alpha 1; targeted mutation 1, Darwin J Prockop
• MGI ID: MGI:2152978
• Gene: Col2a1 Location: Chr15:97873483-97902525 bp, - strand Genetic Position: Chr15, 53.97 cM
• Alliance: Col2a1^tm1Prc page

Abnormal intervertebral disc development in Col2a1^tm1Prc/Col2a1^tm1Prc mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:30041
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 35. RT-PCR analysis demonstrated the presence of a transcript that terminated at exon 35. A mature protein was not detected in cultured cartilage cells isolated from homozygous mice. (J:30041)

Disease models

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	30 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col2a1 Mutation: 68 strains or lines available

References

• Original: J:30041 Li SW, et al., Transgenic mice with targeted inactivation of the Col2 alpha 1 gene for collagen II develop a skeleton with membranous and periosteal bone but no endochondral bone. Genes Dev. 1995 Nov 15;9(22):2821-30
• All: 10 reference(s)