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Targeted Allele Detail
Symbol: Gnai2tm1Lbi
Name: guanine nucleotide binding protein (G protein), alpha inhibiting 2; targeted mutation 1, Lutz Birnbaumer
MGI ID: MGI:2152603
Synonyms: alphai2-, Gai2-, Galphai2-, Galphai2-KO, Gnai2-, Gnai2tm1Uru, Gnao1.2tm1LBi
Gene: Gnai2  Location: Chr9:107614125-107635367 bp, - strand  Genetic Position: Chr9, 58.43 cM
Rib and sternum defects in mice with mutations of Gnai1, Gnai2, and/or Gnai3

Show the 1 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:194592
Parent Cell Line:  AB1 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprt+
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
Mutation detailsA neomycin resistance cassette was inserted into exon 3. The truncated protein is predicted to be inactive and also lack the cysteine at position 352 which is ADP-ribosylated by pertussis toxin. No protein was detected in homogenates from homozygous mice by ADP-ribosylation with pertussis toxin. The absence of protein was also confirmed by immunoblotting. (J:24434, J:194592)
View phenotypes for all genotypes (concatenated display).
Disease models
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gnai2 Mutation:  41 strains or lines available
Original:  J:194592 Rudolph U, et al., Disruption of the G(i2) alpha locus in embryonic stem cells and mice: a modified hit and run strategy with detection by a PCR dependent on gap repair. Transgenic Res. 1993 Nov;2(6):345-55
All:  64 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
MGI 6.03
The Jackson Laboratory