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Ephb2tm2Paw
Targeted Allele Detail
Nomenclature
Symbol: Ephb2tm2Paw
Name: Eph receptor B2; targeted mutation 2, Tony Pawson
MGI ID: MGI:2149670
Synonyms: EphB2ki, EphB2lacZ, EphB2lacZ(kin-), EphB2/LZ, EphB2N2, NuklacZ
Gene: Ephb2  Location: Chr4:136647539-136835988 bp, - strand  Genetic Position: Chr4, 69.0 cM, cytoband D-E
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:34200
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsInsertion of a LacZ-neomycin resistance cassette in-frame at codon 622. Western blot analysis using a polyclonal Ephb2 antibody did not detect protein in cell surface immunoprecipitates of primary brain cultures from homozygous mutant neonates (P4), however a polyclonal beta-galactosidase antibody did detect a novel 200 kDa protein in these cultures. The authors propose that the mutant allele produces a fusion receptor protein comprised of the extracellular, transmembrane, and juxtamembrane domains (amino acids 1-621) linked to beta-galactosidase, and that this mutated receptor lacks the entire tyrosine kinase catalytic and C-terminal domains (amino acids 622-707). (J:34200)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 112 assay results
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ephb2 Mutation:  10 strains or lines available
References
Original:  J:34200 Henkemeyer M, et al., Nuk controls pathfinding of commissural axons in the mammalian central nervous system. Cell. 1996 Jul 12;86(1):35-46
All:  43 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/11/2020
MGI 6.14
The Jackson Laboratory