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Targeted Allele Detail
Symbol: Tnftm1Gkl
Name: tumor necrosis factor; targeted mutation 1, George Kollias
MGI ID: MGI:2149024
Synonyms: TNF-, Tnf0, Tnf-alpha-, TNFalpha-, TNFalpha KO, TNFKO
Gene: Tnf  Location: Chr17:35199381-35202007 bp, - strand  Genetic Position: Chr17, 18.59 cM
Germline Transmission:  Earliest citation of germline transmission: J:47673
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsReplacement of 40 bp of the 5' UTR, all the coding region, including the ATG translation initiation codon, of the first exon and part of the first intron with MC1neopA cassette. Northern blot analysis of LPS stimulated macrophages confirmed disruption of Tnf mRNA expression. (J:47673)
View phenotypes for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 5 strains available      Cell Lines: 0 lines available
Carrying any Tnf Mutation:  20 strains or lines available
Phenotypic Similarity to Human Syndrome: Inflammatory Bowel Disease when compound heterozygote with Tnftm2Gkl (J:54056).

Original:  J:47673 Pasparakis M, et al., Immune and inflammatory responses in TNF alpha-deficient mice: a critical requirement for TNF alpha in the formation of primary B cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response [see comments]. J Exp Med. 1996 Oct 1;184(4):1397-411
All:  303 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
MGI 6.01
The Jackson Laboratory