Tnc^tm1Ref
Targeted Allele Detail

Summary

• Symbol: Tnc^tm1Ref
• Name: tenascin C; targeted mutation 1, Reinhard Fassler
• MGI ID: MGI:2148541
• Gene: Tnc Location: Chr4:63878022-63965252 bp, - strand Genetic Position: Chr4, 34.06 cM
• Alliance: Tnc^tm1Ref page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33828
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a NcoI genomic fragment in exon 2 that contained sequences encoding the signal peptide and the heptad repeat sequences. RT-PCR analysis demonstrated the presence of a shorter aberrant transcript in homozygous mice, but no protein was detectable by Western blot analysis in pooled extracts of cerebellum, lung and thymus. (J:33828)

Expression

In Mice Carrying this Mutation:

42 assay results 2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnc Mutation: 130 strains or lines available

References

• Original: J:33828 Forsberg E, et al., Skin wounds and severed nerves heal normally in mice lacking tenascin-C. Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6594-9
• All: 31 reference(s)