Tnc^tm1Sia
Targeted Allele Detail

Summary

• Symbol: Tnc^tm1Sia
• Name: tenascin C; targeted mutation 1, Shinichi Aizawa
• MGI ID: MGI:2148540
• Synonyms: TN-
• Gene: Tnc Location: Chr4:63878022-63965252 bp, - strand Genetic Position: Chr4, 34.06 cM
• Alliance: Tnc^tm1Sia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36890
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A lacZ gene and neomycin resistance cassette were inserted into exon 2, just before the translation initiation codon. Two thirds of exon 2 and a part of intron 2 were deleted. This allele expresses beta-galactosidase under the control of the endogenous promoter. Northern analysis demonstrated that homozygous mice did not express wild-type mRNA. Immunohistochemistry on thymus, lung and cerebellum failed to detect wild-type protein in homozygous mice. (J:36890, J:51408)

Expression

In Mice Carrying this Mutation:	67 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 11 strains available Cell Lines: 0 lines available
• Carrying any Tnc Mutation: 130 strains or lines available

References

• Original: J:36890 Saga Y, et al., Mice develop normally without tenascin. Genes Dev. 1992 Oct;6(10):1821-31
• All: 62 reference(s)