Ptger4^tm1Bhk
Targeted Allele Detail

Summary

• Symbol: Ptger4^tm1Bhk
• Name: prostaglandin E receptor 4 (subtype EP4); targeted mutation 1, Beverly H Koller
• MGI ID: MGI:2137850
• Synonyms: EP4-
• Gene: Ptger4 Location: Chr15:5262880-5273668 bp, - strand Genetic Position: Chr15, 1.99 cM
• Alliance: Ptger4^tm1Bhk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44108
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1 and 2, which encode the initiation codon and regions of the gene encoding six of the seven transmembrane-spanning domains and putative extracellular loops, were replaced by a neomycin resistance gene. Northern blot analysis of RNA from thymus and spleen of homozygous mutant neonates using cDNA probes specific for this gene verified the loss of expression. (J:44108)

Expression

In Mice Carrying this Mutation:	27 assay results
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptger4 Mutation: 30 strains or lines available

Notes

Phenotypic Similarity to Human Syndrome: Patent Ductus Arteriosus in homozygous mice (J:44108)

References

• Original: J:44108 Nguyen M, et al., The prostaglandin receptor EP4 triggers remodelling of the cardiovascular system at birth. Nature. 1997 Nov 6;390(6655):78-81
• All: 21 reference(s)