Pdgfrb^tm5Sor
Targeted Allele Detail

Summary

• Symbol: Pdgfrb^tm5Sor
• Name: platelet derived growth factor receptor, beta polypeptide; targeted mutation 5, Philippe Soriano
• MGI ID: MGI:2135534
• Synonyms: beta/alphaKI
• Gene: Pdgfrb Location: Chr18:61178222-61218133 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Pdgfrb^tm5Sor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67735
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, No functional change)
• Mutation: Insertion
• Mutation details: A cDNA encoding a chimeric receptor consisting of sequences encoding the extracellular portion of the PDGFbetaR fused to the intracellular portion of the PDGFalphaR was targeted into the Pdgfrb locus. The cDNA was inserted in frame into a Sma1 site in the second coding exon. Western blot analysis indicated that expression of the targeted Pdgfr locus was disrupted and the chimeric receptor was expressed. (J:67735)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdgfrb Mutation: 84 strains or lines available

References

• Original: J:67735 Klinghoffer RA, et al., The two PDGF receptors maintain conserved signaling in vivo despite divergent embryological functions. Mol Cell. 2001 Feb;7(2):343-54
• All: 1 reference(s)