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Targeted Allele Detail
Symbol: Pou5f1tm1Cgre
Name: POU domain, class 5, transcription factor 1; targeted mutation 1, Centre for Genome Research
MGI ID: MGI:1933381
Synonyms: Oct4-
Gene: Pou5f1  Location: Chr17:35506018-35510772 bp, + strand  Genetic Position: Chr17, 18.69 cM
Germline Transmission:  Earliest citation of germline transmission: J:51139
Parent Cell Line:  CGR8 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
Mutation detailsAn internal ribosome entry site-lacZ-neomycin resistance cassette (IRES-beta-geo) replaced sequences in exons 2-5 encoding the two DNA binding domains and the carboxy terminal transactivation site. Immunostaining did not detect wild-type protein in 3 of 15 2.5 day morulae from an intercross of heterozygous mutant animals. (J:51139)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 3 assay results
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pou5f1 Mutation:  75 strains or lines available
Original:  J:51139 Nichols J, et al., Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell. 1998 Oct 30;95(3):379-91
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.12
The Jackson Laboratory