Gt(ROSA)26Sor
Gene trapped Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor
• Name: gene trap ROSA 26, Philippe Soriano
• MGI ID: MGI:1890203
• Synonyms: gtrosa26, R26R3, R26^tg, ROSA26, Rosa26lacZ, ROSA26-lacZ, TgR(ROSA26)26Sor
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor page

Mutation origin

• Mutant Cell Line: FHCRC-GT-ROSA26
• Germline Transmission: Earliest citation of germline transmission: J:79478
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Gene trapped (Not Applicable)
• Mutation: Insertion of gene trap vector Vector: ROSABetageo
• Mutation details: Random insertion of retroviral gene trap vector ROSA, Reverse Orientation with Splice Acceptor, which contains the beta-galactosidase gene and accompanying splice acceptor and neo sequences. The construct was inserted in reverse orientation in the retroviral vector pGen-. The transgene is controlled by an unknown endogenous promoter. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult transgenic mouse. (J:39814, J:79478)
• Inheritance: Not Specified
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  FHCRC-GT-ROSA26	CG465838 ; (MGI Seq Detail)	5' RACE	Chr6:113054028-113054157 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  176 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

6 assay results

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 16 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

Notes

ES cell line = AB1 or AB2.1. No overt phenotypes in heterozygous or homozygous animals. The trapped gene appears to be ubiquitously expressed during embryonic development and in all hematopoietic cells.

References

• Original: J:79478 Friedrich G, et al., Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev. 1991 Sep;5(9):1513-23
• All: 108 reference(s)