Chrna7^tm1Bay
Targeted Allele Detail

Summary

• Symbol: Chrna7^tm1Bay
• Name: cholinergic receptor, nicotinic, alpha polypeptide 7; targeted mutation 1, Baylor College of Medicine
• MGI ID: MGI:1888407
• Synonyms: Acralpha7 -, alpha7, alpha7 NNR -, Chrna7^Baytm1
• Gene: Chrna7 Location: Chr7:62748440-62862274 bp, - strand Genetic Position: Chr7, 34.47 cM
• Alliance: Chrna7^tm1Bay page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44288
• Parent Cell Line: AB2.1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 7 kb genomic fragment containing exons 8-10 was replaced with a neomycin selection cassette. The deleted sequences encode the second through the fourth transmembrane domains and the cytoplasmic loop. Northern blot analysis on brain samples derived from homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on brain extracts derived from homozygous mice also confirmed that no detectable protein was made from this allele. (J:44288, J:122939)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrna7 Mutation: 49 strains or lines available

References

• Original: J:44288 Orr-Urtreger A, et al., Mice deficient in the alpha7 neuronal nicotinic acetylcholine receptor lack alpha-bungarotoxin binding sites and hippocampal fast nicotinic currents. J Neurosci. 1997 Dec 1;17(23):9165-71
• All: 188 reference(s)