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Apctm1Tno
Targeted Allele Detail
Nomenclature
Symbol: Apctm1Tno
Name: APC, WNT signaling pathway regulator; targeted mutation 1, Tetsuo Noda
MGI ID: MGI:1857966
Synonyms: 580S, Apc580s, Apc580S flox, Apcf, Apcfl, ApcloxP, Apclx
Gene: Apc  Location: Chr18:34220924-34322552 bp, + strand  Genetic Position: Chr18, 18.53 cM
Anterior head defects during embryonic development in ApcMin/Apctm1Tno embryos and rescue of head defects by Ctnnb1tm4.1Wbm/Ctnnb1+

Show the 3 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:43301
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Conditional ready, Hypomorph)
Mutation:    Insertion
 
Mutation detailsOne loxP site was inserted into intron 13 and the other, with a PGK-neo cassette, into intron 14. Northern blot analysis revealed that RNA expression in the intestinal tract of homozygous mutants was about 30% of that in wild-type mice. Protein expression in the colon of homozygotes by immunohistochemistry was indistinguishable from that of wild-type mice. Western blot analysis of lysates from mouse embryo fibroblasts showed reduced expression of the protein. (J:43301, J:156864)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 13 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 5 strains available      Cell Lines: 0 lines available
Carrying any Apc Mutation:  58 strains or lines available
References
Original:  J:43301 Shibata H, et al., Rapid colorectal adenoma formation initiated by conditional targeting of the Apc gene. Science. 1997 Oct 3;278(5335):120-3
All:  140 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/11/2020
MGI 6.14
The Jackson Laboratory