Bmp8b^tm1Blh
Targeted Allele Detail

Summary

• Symbol: Bmp8b^tm1Blh
• Name: bone morphogenetic protein 8b; targeted mutation 1, Brigid L Hogan
• MGI ID: MGI:1857954
• Gene: Bmp8b Location: Chr4:122998101-123019887 bp, + strand Genetic Position: Chr4, 57.38 cM
• Alliance: Bmp8b^tm1Blh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34057
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 7 kb genomic fragment containing exon 4 was replaced with a neomycin selection cassette. The deleted sequences encode the conserved dibasic proteolytic cleavage site between the pro- and mature regions of the encoded protein. In situ hybridization experiments on sections of testis of homozygous male mice confirmed that no detectable transcript was produced from this allele. (J:34057)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bmp8b Mutation: 26 strains or lines available

Notes

ES cell line = R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl⁺) or TL1 (129S6/SvEvTac).

References

• Original: J:34057 Zhao GQ, et al., The gene encoding bone morphogenetic protein 8B is required for the initiation and maintenance of spermatogenesis in the mouse. Genes Dev. 1996 Jul 1;10(13):1657-69
• All: 8 reference(s)