Prkdc^tm1Gcl
Targeted Allele Detail

Summary

• Symbol: Prkdc^tm1Gcl
• Name: protein kinase, DNA activated, catalytic polypeptide; targeted mutation 1, Gloria C Li
• MGI ID: MGI:1857934
• Synonyms: DNA-PKcs-
• Gene: Prkdc Location: Chr16:15455730-15660099 bp, + strand Genetic Position: Chr16, 10.09 cM, cytoband B1
• Alliance: Prkdc^tm1Gcl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53765
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Half of exon 3 and part of intron 3 was replaced with a PGK-neo cassette via homologous recombination. RT-PCR showed deletion of exons 1-4 in homozygous mutant animals and Western blot failed to detect protein product. In vitro kinase assay of fibroblasts from homozygous mutant animals was negative for kinase activity. (J:53765)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Prkdc^tm1Gcl

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkdc Mutation: 408 strains or lines available

References

• Original: J:53765 Kurimasa A, et al., Catalytic subunit of DNA-dependent protein kinase: impact on lymphocyte development and tumorigenesis. Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1403-8
• All: 8 reference(s)