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Hnf1btm1Mya
Targeted Allele Detail
Nomenclature
Symbol: Hnf1btm1Mya
Name: HNF1 homeobox B; targeted mutation 1, Moshe Yaniv
MGI ID: MGI:1857866
Synonyms: vHNF1-, vHNF1/lacZ
Gene: Hnf1b  Location: Chr11:83850209-83905917 bp, + strand  Genetic Position: Chr11, 51.23 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:58080
Parent Cell Line:  CK35 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsExon 1 was replaced by the insertion of NLS-lacZ and neo. Western blot analysis of total RNA isolated from homozygous differentiated ES cells revealed an absence of transcript. Expression of beta-galactosidase was controlled by the endogenous promoter. (J:58080)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 18 assay results
In Structures Affected by this Mutation: 12 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hnf1b Mutation:  3 strains or lines available
Notes
Complementation of Tcf2tm1Mya mutant embryos with wild-type visceral endoderm by tetraploid aggregation extends viability to E10 and rescues gastrulation and the growth defect up to E9.5; however, at E9.5-E10.0, tetraploid-rescued embryos are shorter and resemble E8.5 embryos. Additional phenotypes include: incomplete embryo turning, failure to close the neural tube, neural tissue overgrowth associated with edema on both neural folds and on forebrain vesicles, ruffling of the visceral endoderm, an enlarged allantois, distended pericardia, and failure to close and/or invaginate the gut (J:58080). Injection of mutant ES cells into wild-type blastocysts results in chimeric animals with a closed neural tube and a normal mesonephros; however, mutant ES cells fail to contribute to gut formation, as shown by absence of X-gal staining (J:58080).
References
Original:  J:58080 Coffinier C, et al., Essential role for the homeoprotein vHNF1/HNF1beta in visceral endoderm differentiation. Development. 1999 Nov;126(21):4785-94
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/11/2020
MGI 6.14
The Jackson Laboratory