Hnf1b^tm1Mya
Targeted Allele Detail

Summary

• Symbol: Hnf1b^tm1Mya
• Name: HNF1 homeobox B; targeted mutation 1, Moshe Yaniv
• MGI ID: MGI:1857866
• Synonyms: vHNF1^-, vHNF1/lacZ
• Gene: Hnf1b Location: Chr11:83741035-83796743 bp, + strand Genetic Position: Chr11, 51.23 cM
• Alliance: Hnf1b^tm1Mya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58080
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was replaced by the insertion of NLS-lacZ and neo. Western blot analysis of total RNA isolated from homozygous differentiated ES cells revealed an absence of transcript. Expression of beta-galactosidase was controlled by the endogenous promoter. (J:58080)

Expression

In Mice Carrying this Mutation:	18 assay results
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hnf1b Mutation: 16 strains or lines available

Notes

Complementation of Tcf2^tm1Mya mutant embryos with wild-type visceral endoderm by tetraploid aggregation extends viability to E10 and rescues gastrulation and the growth defect up to E9.5; however, at E9.5-E10.0, tetraploid-rescued embryos are shorter and resemble E8.5 embryos. Additional phenotypes include: incomplete embryo turning, failure to close the neural tube, neural tissue overgrowth associated with edema on both neural folds and on forebrain vesicles, ruffling of the visceral endoderm, an enlarged allantois, distended pericardia, and failure to close and/or invaginate the gut (J:58080). Injection of mutant ES cells into wild-type blastocysts results in chimeric animals with a closed neural tube and a normal mesonephros; however, mutant ES cells fail to contribute to gut formation, as shown by absence of X-gal staining (J:58080).

References

• Original: J:58080 Coffinier C, et al., Essential role for the homeoprotein vHNF1/HNF1beta in visceral endoderm differentiation. Development. 1999 Nov;126(21):4785-94
• All: 7 reference(s)