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Vcltm1Eda
Targeted Allele Detail
Nomenclature
Symbol: Vcltm1Eda
Name: vinculin; targeted mutation 1, Eileen D Adamson
MGI ID: MGI:1857730
Synonyms: Vcl0, vinculin-
Gene: Vcl  Location: Chr14:20929433-21033673 bp, + strand  Genetic Position: Chr14, 11.53 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:45781
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsExon E3 was replaced by a neomycin cassette. Homozygous mutant embryos do not express vinculin protein, as assesed by Western blotting. The strategy employed to inactivate the vinculin gene leads to impaired production of vinculin as well as metavinculin. Heterozygotes exhibit a 58% reduction of vinculin and a 63% reduction of its muscle splice variant (metavinculin) protein levels versus wild-type littermates. (J:45781, J:92398)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 6 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Vcl Mutation:  10 strains or lines available
References
Original:  J:45781 Xu W, et al., Vinculin knockout results in heart and brain defects during embryonic development. Development. 1998 Jan;125(2):327-37
All:  3 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
08/25/2015
MGI 6.0
The Jackson Laboratory