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Nkx2-5tm1Siz
Targeted Allele Detail
Nomenclature
Symbol: Nkx2-5tm1Siz
Name: NK2 homeobox 5; targeted mutation 1, Seigo Izumo
MGI ID: MGI:1857618
Synonyms: Csx/Nkx2.5-, Nkx2-5lacZ, Nkx2-5Lz
Gene: Nkx2-5  Location: Chr17:26838664-26841565 bp, - strand  Genetic Position: Chr17, 13.6 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:52597
Parent Cell Line:  AK7 (ES Cell)
Strain of Origin:  129S4/SvJaeSor
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
    A lacZ-PGK-neomycin resistance cassette replaced all of the coding sequences. (J:52597)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 79 assay results
In Structures Affected by this Mutation: 12 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nkx2-5 Mutation:  15 strains or lines available
Notes
Homozygous mutant ES cells were injected into C57BL/6 blastocysts to form chimeric animals. Mutant ES cells were shown to contribute to most tissues of surviving animals except heart. Embryos with more than 30-40% contribution of mutant cells to the heart exhibit the phenotype of the homozygous mutant. Embryos with a 20-30% contribution of mutant ES cells to the heart exhibited milder phenotypic defects than the homozygous mutant. Embryos with less than 15% contribution of mutant cells to the heart exhibit no significant heart defects at E10.5(J:52597).
References
Original:  J:52597 Tanaka M, et al., The cardiac homeobox gene Csx/Nkx2.5 lies genetically upstream of multiple genes essential for heart development. Development. 1999 Mar;126(6):1269-80
All:  19 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
10/16/2018
MGI 6.12
The Jackson Laboratory