Gck^tm1Ts
Targeted Allele Detail

Summary

• Symbol: Gck^tm1Ts
• Name: glucokinase; targeted mutation 1, Timothy Stewart
• MGI ID: MGI:1857469
• Synonyms: GKKO, GLK-
• Gene: Gck Location: Chr11:5850820-5900081 bp, - strand Genetic Position: Chr11, 3.88 cM
• Alliance: Gck^tm1Ts page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29255
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced all of exon 4 and parts of exons 3 and 5 of the gene. Northern blots of liver from heterozygous and homozygous mutant mice showed decreased levels of mRNA and no detectable mRNA, respectively, for the gene of interest. Enzymatic activity in both pancreatic islet cells and liver from heterozygous mutant mice was approximately 60% of wild-type littermates. Enzymatic activity in liver from homozygous mutant mice was undetectable. (J:29255)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gck Mutation: 59 strains or lines available

References

• Original: J:29255 Grupe A, et al., Transgenic knockouts reveal a critical requirement for pancreatic beta cell glucokinase in maintaining glucose homeostasis. Cell. 1995 Oct 6;83(1):69-78
• All: 2 reference(s)