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Targeted Allele Detail
Symbol: Nos3tm1Unc
Name: nitric oxide synthase 3, endothelial cell; targeted mutation 1, University of North Carolina
MGI ID: MGI:1857229
Synonyms: ecNOS-, eNOS-, eNOSKO, NOS3-
Gene: Nos3  Location: Chr5:24364810-24384474 bp, + strand  Genetic Position: Chr5, 11.32 cM
Germline Transmission:  Earliest citation of germline transmission: J:36559
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA 1.2 kb neomycin cassette replaced 129 bp of exon 12 of the gene. This disrupted the calmodulin binding site of the protein and introduced a premature stop codon into the transcripts. Immunohistochemisty of heart and kidney sections from homozygous mutant mice confirmed that no detectable encoded protein was present. (J:36559)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 6 strains available      Cell Lines: 0 lines available
Carrying any Nos3 Mutation:  30 strains or lines available
Original:  J:36559 Shesely EG, et al., Elevated blood pressures in mice lacking endothelial nitric oxide synthase. Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13176-81
All:  321 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.11
The Jackson Laboratory