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Targeted Allele Detail
Symbol: Nos3tm1Unc
Name: nitric oxide synthase 3, endothelial cell; targeted mutation 1, University of North Carolina
MGI ID: MGI:1857229
Synonyms: ecNOS-, eNOS-, eNOSKO, NOS3-
Gene: Nos3  Location: Chr5:24364810-24384474 bp, + strand  Genetic Position: Chr5, 11.32 cM
Germline Transmission:  Earliest citation of germline transmission: J:36559
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA 1.2 kb neomycin cassette replaced 129 bp of exon 12 of the gene. This disrupted the calmodulin binding site of the protein and introduced a premature stop codon into the transcripts. Immunohistochemisty of heart and kidney sections from homozygous mutant mice confirmed that no detectable encoded protein was present. (J:36559)
View phenotypes for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 6 strains available      Cell Lines: 0 lines available
Carrying any Nos3 Mutation:  7 strains or lines available
Phenotypic Similarity to Human Syndrome: Aortic Valve Disease in Homozygous mice (J:103340)

Original:  J:36559 Shesely EG, et al., Elevated blood pressures in mice lacking endothelial nitric oxide synthase. Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13176-81
All:  298 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.0
The Jackson Laboratory