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Drd3tm1Dac
Targeted Allele Detail
Nomenclature
Symbol: Drd3tm1Dac
Name: dopamine receptor D3; targeted mutation 1, Domenico Accili
MGI ID: MGI:1857159
Synonyms: D3-, delta148(+30)
Gene: Drd3  Location: Chr16:43762221-43822932 bp, + strand  Genetic Position: Chr16, 28.44 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:32128
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsA neomycin selection cassette and sequence encoding 30 non-endogenous residues was inserted into exon 2. RT-PCR analysis on RNA derived from brain of homozygous mice demonstrated the absence of normal transcript. Mutant transcript was detected in both heterozygous and homozygous mutant mice. Binding assays on brain sections derived from homozygous mice confirmed that no functional protein was expressed. Notably, heterozygotes displayed a nearly complete loss of D3-specific binding, to a greater extent than expected for a mutation affecting expression of one allele of the Drd3 gene, raising the possibility that this mutation acts in a dominant-negative fashion. (J:32128)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Drd3 Mutation:  39 strains or lines available
References
Original:  J:32128 Accili D, et al., A targeted mutation of the D3 dopamine receptor gene is associated with hyperactivity in mice. Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1945-9
All:  36 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
11/14/2017
MGI 6.11
The Jackson Laboratory