Adra2b^tm1Gsb
Targeted Allele Detail

Summary

• Symbol: Adra2b^tm1Gsb
• Name: adrenergic receptor, alpha 2b; targeted mutation 1, Gregory S Barsh
• MGI ID: MGI:1857118
• Synonyms: alpha2B-, alpha2B-KO
• Gene: Adra2b Location: Chr2:127205128-127209141 bp, + strand Genetic Position: Chr2, 61.95 cM
• Alliance: Adra2b^tm1Gsb page

Dilated right ventricle in Adra2b^tm1Gsb/Adra2b^tm1Gsb mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34819
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into the coding sequences, placing a premature stop codon before the sequences encoding the fifth transmembrane domain. Western blot analysis on kidney membrane extracts confirmed that the protein was not detectable in homozygous mice. (J:34819)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Adra2b Mutation: 22 strains or lines available

References

• Original: J:34819 Link RE, et al., Cardiovascular regulation in mice lacking alpha2-adrenergic receptor subtypes b and c. Science. 1996 Aug 9;273(5276):803-5
• All: 23 reference(s)