Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Trpa1tm1.1Kykw
Name:	transient receptor potential cation channel, subfamily A, member 1; targeted mutation 1.1, Kelvin Y Kwan
MGI ID:	MGI:5532838
Gene:	Trpa1  Location: Chr1:14942872-14989086 bp, - strand  Genetic Position: Chr1, 4.5 cM
Alliance:	Trpa1tm1.1Kykw page

Germline Transmission:	Earliest citation of germline transmission: J:108343
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutation:    Insertion   Mutation details: A targeting vector was created to replace exons 21-24 (encoding the pore forming domain of the gene) with an endoplasmic reticulum retention signal (peptide KDEL), an internal ribosome entry site (IRES), a human placental alkaline phosphatase gene (PLAP), and a polyadenylation sequence (pA) followed by an FRT-flanked neomycin resistance cassette. FLP-mediated recombination removed the neo cassette. (J:108343)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Trpa1 Mutation:	92 strains or lines available

Original:	J:108343 Kwan KY, et al., TRPA1 contributes to cold, mechanical, and chemical nociception but is not essential for hair-cell transduction. Neuron. 2006 Apr 20;50(2):277-89
All:	22 reference(s)