Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Usp2tm1.1Bjc
Name:	ubiquitin specific peptidase 2; targeted mutation 1.1, Joseph C Besharse
MGI ID:	MGI:5302030
Gene:	Usp2  Location: Chr9:43978318-44006924 bp, + strand  Genetic Position: Chr9, 24.53 cM, cytoband B
Alliance:	Usp2tm1.1Bjc page

Germline Transmission:	Earliest citation of germline transmission: J:177916
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A floxed neo cassette was inserted upstream of exon 3 and a loxP site was inserted downstream of exon 4 via homologous recombination. Cre mediated recombination removed the neo cassette and exons 3 - 4. Western blot analysis of total retina lysate from homozygous mice confirmed the absence of the 69 kDa isoform but did detect low levels of expression at about 45 kDa. RT_PCR analysis also detected low levels of expression of a short transcript. Sequencing determined this transcript was formed by alternative splicing of exon 1 to exon 5. (J:177916)

Generation of the Usp2tm1Bjc and Usp2tm1.1Bjc alleles

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Usp2 Mutation:	32 strains or lines available

Original:	J:177916 Scoma HD, et al., The de-ubiquitinylating enzyme, USP2, is associated with the circadian clockwork and regulates its sensitivity to light. PLoS One. 2011;6(9):e25382
All:	1 reference(s)