Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Fgf8tm2.1Jyhl
Name:	fibroblast growth factor 8; targeted mutation 2.1, James Y H Li
MGI ID:	MGI:4437219
Synonyms:	Fgf8deltaa
Gene:	Fgf8  Location: Chr19:45725237-45731354 bp, - strand  Genetic Position: Chr19, 38.75 cM, cytoband C3-D
Alliance:	Fgf8tm2.1Jyhl page

Germline Transmission:	Earliest citation of germline transmission: J:156717
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A point mutation into the 3' alternative splice acceptor of Fgf8 exon 1D, changing the sequence from cagCATGTG to caaCATGTG. A floxed neo selection cassette was place 400 bp downstream of exon 1D. The cassette was removed by cre mediated recombination. RT-PCR demonstrated that the transcripts of Fgf8a-containing spliceforms (a, c, e, and g) were missing, while Fgf8b-containing spliceforms, which utilize the remaining alternative splice acceptor of the exon 1D, are expressed. (J:156717)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Fgf8 Mutation:	18 strains or lines available

Original:	J:156717 Guo Q, et al., Fgf8b-containing spliceforms, but not Fgf8a, are essential for Fgf8 function during development of the midbrain and cerebellum. Dev Biol. 2010 Feb 15;338(2):183-92
All:	1 reference(s)