Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Kitltm3.1Pbes
Name:	kit ligand; targeted mutation 3.1, Peter Besmer
MGI ID:	MGI:4430868
Synonyms:	KitLdelta9
Gene:	Kitl  Location: Chr10:99851492-99936278 bp, + strand  Genetic Position: Chr10, 51.4 cM
Alliance:	Kitltm3.1Pbes page

Germline Transmission:	Earliest citation of germline transmission: J:157170
Parent Cell Line:	CJ7 (ES Cell)
Strain of Origin:	129S1/Sv-Oca2+ Tyr+ Kitl+

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A neomycin resistance cassette replaced exon 9, which encodes the C-terminal tail sequence. Splicing resulted in addition of 23 extraneous amino acids following exon 8 sequence. The absence of exon 9 was confirmed by sequencing. The floxed neomycin cassette was excised in vivo by Cre mediated recombination. Western blot analysis using extracts from the cerebellum showed that the total protein expression levels were similar to wild-type mice. ELISA showed a two-fold reduction of soluble protein levels in the plasma. (J:157170)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Kitl Mutation:	92 strains or lines available

Original:	J:157170 Deshpande S, et al., Kit ligand cytoplasmic domain is essential for basolateral sorting in vivo and has roles in spermatogenesis and hematopoiesis. Dev Biol. 2010 Jan 15;337(2):199-210
All:	1 reference(s)