Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Gucy1a1tm1.1Brou
Name:	guanylate cyclase 1, soluble, alpha 1; targeted mutation 1.1, Peter Brouckaert
MGI ID:	MGI:4354143
Synonyms:	sGCalph1-
Gene:	Gucy1a1  Location: Chr3:81999734-82053096 bp, - strand  Genetic Position: Chr3, 35.97 cM
Alliance:	Gucy1a1tm1.1Brou page

Germline Transmission:	Earliest citation of germline transmission: J:151161
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: Exon 6 of Gucy1a3 gene which codes for a conserved portion of the catalytic domain was flanked by a loxP and a loxP-neo-loxP cassette. The exon 6 and neo cassette was removed in ES cells by transient cre expression leaving a single loxP in place. Northern blot and RT-PCR confirmed excision of exon 6 in the transcript; however, immunoblot revealed a smaller mutant 59-kDa protein in homozygous mice. Mutant protein was determined to be inactive, thus it is functional null allele. (J:151161)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	11 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gucy1a1 Mutation:	40 strains or lines available

Original:	J:113106 Cauwels A, et al., Anaphylactic shock depends on PI3K and eNOS-derived NO. J Clin Invest. 2006 Aug;116(8):2244-51
All:	16 reference(s)