Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Seletm2Hyn
Name:	selectin, endothelial cell; targeted mutation 2, Richard Hynes
MGI ID:	MGI:3839963
Synonyms:	E-selectin-
Gene:	Sele  Location: Chr1:163875773-163885246 bp, + strand  Genetic Position: Chr1, 71.35 cM
Alliance:	Seletm2Hyn page

Germline Transmission:	Earliest citation of germline transmission: J:31626
Parent Cell Line:	D3 (ES Cell)
Strain of Origin:	129S2/SvPas

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A modified D3 ES cell line heterozygous for the Selptm1Hyn allele was targeted a second time. The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. Both Northern and RT-PCR analysis revealed an absence of normal mRNA for E-selectin but the presence of mRNA for P-selectin in extracts from cardiac and pulmonary tissue of homozygous mutant mice. This targeted mutation occurred in trans with the Selptm1Hyn mutation and were subsequently bred apart. (J:31626)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Sele Mutation:	51 strains or lines available

Original:	J:31626 Frenette PS, et al., Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell. 1996 Feb 23;84(4):563-74
All:	15 reference(s)