Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tpsb2tm1.1Mfg
Name:	tryptase beta 2; targeted mutation 1.1, Michael F Gurish
MGI ID:	MGI:3783331
Synonyms:	Mcpt6-, mMCP-6-, Tpsb2tm1Dle
Gene:	Tpsb2  Location: Chr17:25585282-25587070 bp, + strand  Genetic Position: Chr17, 12.53 cM, cytoband A3.3
Alliance:	Tpsb2tm1.1Mfg page

Germline Transmission:	Earliest citation of germline transmission: J:133373
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129/Sv

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: Parts of exon 2 and 3 were replaced with an EGFP expression cassette followed by two polyA stop sequences and a loxP-flanked neomycin resistance cassette. Founder mice were mated with cre transgenic mice to remove the neomycin selection cassette. Gene inactivation was confirmed by immunoblot analysis on bone marrow derived mast cells. Immunoblot analysis also confirmed that the adjacent Tpsab1 gene locus contained the intact 129 allele and not the null C57BL/6 allele. (J:133373)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tpsb2 Mutation:	12 strains or lines available

Original:	J:133373 Shin K, et al., Mouse Mast Cell Tryptase mMCP-6 Is a Critical Link between Adaptive and Innate Immunity in the Chronic Phase of Trichinella spiralis Infection. J Immunol. 2008 Apr 1;180(7):4885-4891
All:	9 reference(s)