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Rapgef6tm1.1Tkat
Targeted Allele Detail
Summary
Symbol: Rapgef6tm1.1Tkat
Name: Rap guanine nucleotide exchange factor (GEF) 6; targeted mutation 1.1, Tohru Kataoka
MGI ID: MGI:3760543
Synonyms: RA-GEF-2-
Gene: Rapgef6  Location: Chr11:54413673-54590111 bp, + strand  Genetic Position: Chr11, 32.13 cM
Alliance: Rapgef6tm1.1Tkat page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:126164
Parent Cell Line:  EB3 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation details: Exon 21 was removed by cre-mediated deletion following a cross to Tg(ACTB-cre)13Miya mice to create an out of frame deletion. Absence of protein product was confirmed by western blot on protein extracts from lung, thymus, brain, spleen and pancreas. (J:126164)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Rapgef6 Mutation:  110 strains or lines available
Notes
ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:126164 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.

Phenotypic Similarity to Human Syndrome: Oligoasthenoteratozoospermia (OAT) Syndrome J:218581
References
Original:  J:126164 Yoshikawa Y, et al., The M-Ras-RA-GEF-2-Rap1 pathway mediates tumor necrosis factor-alpha dependent regulation of integrin activation in splenocytes. Mol Biol Cell. 2007 Aug;18(8):2949-59
All:  6 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory