Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Cd28tm1Shr
Name:	CD28 antigen; targeted mutation 1, Arlene H Sharpe
MGI ID:	MGI:3721239
Synonyms:	Cd28-
Gene:	Cd28  Location: Chr1:60785547-60812521 bp, + strand  Genetic Position: Chr1, 30.52 cM, cytoband C1-C3
Alliance:	Cd28tm1Shr page

Germline Transmission:	Earliest citation of germline transmission: J:68642
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A PGK-hygro cassette replaced a 3 kb region encompassing portions of exon 2 and exon 3. The deleted region encoded parts of a IgV-like domain and a transmembrane domain. Fluorescence-activated cell sorting (FACS) analysis showed an absence of protein on naive CD4 T cells isolated from the lymph nodes of homozygous mutant mice. Targeting was performed on an embryonic stem cell clone that was heterozygote for a targeted mutation of Ctla4tm1Shr. (J:68642)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Cd28 Mutation:	53 strains or lines available

Original:	J:68642 Mandelbrot DA, et al., B7-dependent T-cell costimulation in mice lacking CD28 and CTLA4. J Clin Invest. 2001 Apr;107(7):881-7
All:	5 reference(s)