Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Atp2a2tm1Fwuy
Name:	ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; targeted mutation 1, Frank Wuytack
MGI ID:	MGI:3653502
Synonyms:	Serca2a-, SERCA2b
Gene:	Atp2a2  Location: Chr5:122591576-122640288 bp, - strand  Genetic Position: Chr5, 62.38 cM, cytoband F
Alliance:	Atp2a2tm1Fwuy page

Germline Transmission:	Earliest citation of germline transmission: J:111247
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A targeting vector was used to insert an inactivated muscle-specific 5' donor splice site and a strong polyA site. A neomycin resistance cassette replaced untranslated exons 23 and 24, and the muscle-specific exon 25. The resulting allele lacked expression of the protein in the heart and prevented its replacement by the b isoform. RNase protection assay and Western blot failed to detect transcript and protein, respectively, in mutants. (J:111247)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

8 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Atp2a2 Mutation:	76 strains or lines available

Original:	J:111247 Ver Heyen M, et al., Replacement of the muscle-specific sarcoplasmic reticulum Ca(2+)-ATPase isoform SERCA2a by the nonmuscle SERCA2b homologue causes mild concentric hypertrophy and impairs contraction-relaxation of the heart. Circ Res. 2001 Oct 26;89(9):838-46
All:	6 reference(s)