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Trhtm1Mmt
Targeted Allele Detail
Summary
Symbol: Trhtm1Mmt
Name: thyrotropin releasing hormone; targeted mutation 1, Makoto M Taketo
MGI ID: MGI:3620126
Synonyms: TRH KO
Gene: Trh  Location: Chr6:92219042-92221631 bp, - strand  Genetic Position: Chr6, 41.03 cM
Alliance: Trhtm1Mmt page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:43657
Parent Cell Line:  TT2 (ES Cell)
Strain of Origin:  (C57BL/6NCrlj x CBA/JNCrlj)F1
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation details: A neomycin selection cassette replaced sequences containing codons 70-211. Northern blot analysis indicated that no detectable transcript was expressed from this allele in hypothalamus, and radioimmunoassy experiments confirmed the absence of encoded protein from homozygous mice. (J:43657)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Trh Mutation:  22 strains or lines available
Notes
Mice homozygous for a targeted disruption of Trh are viable, fertile, and develop normally. They exhibit, however, a tertiary hypothyroidism with elevated TSH levels, suggesting a direct effect of TRH on pituitary thyrotrophs. Impaired insulin secretion and hyperglycemia also occur in these mice (J:43657).
References
Original:  J:43657 Yamada M, et al., Tertiary hypothyroidism and hyperglycemia in mice with targeted disruption of the thyrotropin-releasing hormone gene. Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10862-7
All:  9 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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04/16/2024
MGI 6.23 		The Jackson Laboratory