Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Hbegftm3Mek
Name:	heparin-binding EGF-like growth factor; targeted mutation 3, Eisuke Mekada
MGI ID:	MGI:2682961
Synonyms:	HBdelta-tm
Gene:	Hbegf  Location: Chr18:36637980-36648858 bp, - strand  Genetic Position: Chr18, 19.46 cM
Alliance:	Hbegftm3Mek page

Germline Transmission:	Earliest citation of germline transmission: J:86547
Parent Cell Line:	TT2 (ES Cell)
Strain of Origin:	(C57BL/6NCrlj x CBA/JNCrlj)F1

Allele Type:    Targeted (Null/knockout) Mutation:    Insertion   Mutation details: A stop codon was introduced in the cDNA sequence between the codons for amino acid residue 148 and 149. The modified cDNA sequence was then fused to the start codon of exon 1 along with IRES, a neomycin resistance gene and a polyadenylation sequence. RT-PCR of skin and heart specimens confirmed the presence of the modified mRNA transcript. The predicted soluble protein product was detected immunologically. (J:86547)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hbegf Mutation:	25 strains or lines available

Original:	J:86547 Yamazaki S, et al., Mice with defects in HB-EGF ectodomain shedding show severe developmental abnormalities. J Cell Biol. 2003 Nov 10;163(3):469-75
All:	1 reference(s)